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Why does the package insert recommend putting line diluent between Cal F and the first control sample?

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Why does the package insert recommend putting line diluent between Cal F and the first control sample?

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The line diluent was put in place, only as a precaution because the low control is immediately following a high calibrator. A sample carryover study was performed per recommendations in the TDx user’s manual. This study did not show any trends in samples carryover. I there concerned about carryover, each lab should individually perform the sample carryover study.

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