What types of cells are present?
Sketch each type and determine the approximate percentage of the different types. If any of the cell types seen in Step 8 have disappeared, explain what happened to them. 13. Make a subculture by transferring some of the mating mixture with a sterile toothpick to a fresh YED medium agar plate. a. Streak the cells in one horizontal line across the middle of the plate. b. Label this subculture Plate 4 and add your name and the date. 14. Invert the plate and incubate at 30C overnight. Day 4 15. On a plate of unknown medium, make several thick streaks of the freshly grown subculture from Plate 4. Label this culture Plate 5 and add your name and the date. 16. Invert the plate and incubate at 30C for at least 3 days. Day 7 17. Make a wet mount slide of the subculture from Plate 5, observe the cells through the microscope, sketch them, and answer the following questions: What cell types are present now that were not present before? Sketch these cell types. Compare these cell types with the ce
