What is the relationship between structure (phylogeny) and function in the bacterial communities from the different environmental regimes?
We will expand this question from the detailed exploration of diversity within the Prochlorococcus populations to the co-existing uncultured bacterial community by two new approaches. First, we will use our newly developed capture and walk technique that allows us to use oligonucleotide probes specific for a ribotype to pull large genome fragments (up to 20 kb) from the environment. These can be cloned and sequenced, and probes complementary to their ends can be designed for capture of contiguous fragments. Thus, clone libraries that are samples of the co-existing diversity within identical and similar ribotypes can be assembled and the diversity of associated genes explored. Second, we will adapt the in situ amplification technology to a functional multiplexing in which co-localization of specific structural and phylogenetic marker genes can be identified in a high throughput manner. We will concentrate on uncultured bacterial ribotypes found to be either numerically dominant or to be
Related Questions
- What is the relationship between structure (phylogeny) and function in the bacterial communities from the different environmental regimes?
- What is the structure of a bacterial cell and how are they different from other microorganisms?
- How are DNA and RNA similar and different in terms of structure and function?
