What is GreenScreen GC?
GreenScreen GC is the name of our yeast cell-based screening assay for genotoxicity and cytotoxicity. A single microplate is sufficient for the simultaneous testing of 4 compounds over 9 serial dilutions. The protocol is also readily automated using standard laboratory equipment. A key advantage of using yeast is that aliquots of the culture can be stored in a laboratory freezer and are easily resuscitated ready for use after a 72 hour (i.e. over weekend) incubation. The stock culture can be used immediately for assays and remaining stocks are disposed of after 1 week. There is no requirement for on-going passage and maintenance of cell lines, and thus the assay can be handled in a basic laboratory setting. It has a lower specificity and sensitivity than GreenScreen HC.
