What can I use as positive control lysates for BD Transduction Laboratories™ antibodies?
To get the best Western Blotting performance from your BD Transduction Laboratories™ antibody, please use the positive control lysate recommended on our Technical Data Sheets. This will help save you time, sample and antibody. The positive control will help you duplicate our established results in initial experiments by precisely following our established protocols. For detailed protocols please see our website, bdbiosciences.com/support/resources/. We recommend these lysates to be kept at -20°C for long-term storage. Lysates are extremely stable since they are a cell preparations in which all enzymes are denatured and inactivated. We have done extensive research and confirmed stability under different conditions. For example, the integrity and quality of most lysates were not affected by repeated freeze-thaw cycles, or storage at 25°C for up to 10 weeks. The lysates, however, started to show degradation by four weeks at 37°C. There may, however, be some slight variation in stability d
