In the Graphic Analysis Display (GAD), how do I distinguish between real and unreal mutations in the electropherograms?
Scroll through each mutation and delete those that only appear in one direction (Forward or Reverse) and not in both directions. Mutations that appear in both directions and are written in blue are most likely real and should be kept. If the quality of the sample lane is poor (0-10) and a 1 directional mutation is written in red, either delete the mutation or the entire lane by right clicking on Lane Quality.