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How many cells should I add to each well? What happens if I add the cells before adding the antigen or mitogen?

April 26, 2017antigen cells happens mitogen

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How many cells should I add to each well? What happens if I add the cells before adding the antigen or mitogen?

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We recommend adding 3×105 to 1×106 cells per well, depending on the experiment. If cells are added prior to the antigen or mitogen, the spots may be distributed unevenly, to the edges of the wells.