How does HIC work?
In HIC, proteins bind to a hydrophobic ligand on the surface of a porousl methacrylate support resin under high salt concentration conditions in the mobile phase (typically >1M ammonium sulfate). The salt promotes the hydrophobic interaction between the protein and the solid support. To desorb the protein, the salt concentration is lowered via a decreasing salt gradient which diminishes the hydrophobic interaction.
Related Questions
- As a CSL, do I need a HIC? If I have an unrestricted Construction Supervisor License (CSL) do I need to be registered as a Home Improvement Contractor (HIC) for example to add a deck to a 2-Family owner occupied house?
- Do the new CSL Specialty Licenses only cover work formerly covered by a Home Improvement Contractor (HIC) Registration?
- Do I need a CSL or HIC to work on existing owner occupied houses with 1- to 4-dwelling units?
