Does the DR work with Green Fluorescent Protein?
GFP has 2 excitation peaks at 395 and 470 nm. The 470 nm peak is maximally excited by the Dark Reader and the red-shifted variants (e. g., EGFP and EYFP from ClonTech) fluoresce superbly when illuminated with DR light. In addition, the DR does not cause the rapid photobleaching of GFP that occurs with exposure to UV. Visualization of wild-type GFP is usually not practical using a Dark Reader.
