Are transcription factor binding results from the Illumina ChIP Sequencing output quantitative or qualitative?
Measurements of TF binding should be considered semi-quantitative due to amplification of the ChIP DNAs prior to sequencing. Genpathway makes every effort to carry out the amplification in as linear a fashion as possible, although some bias in amplification efficiencies may occur between different types of sequences. When differential binding levels have been validated using Genpathway’s Query assay, the differential binding observed between samples or between sites agreed very well with the ChIP Sequencing numbers, demonstrating good quantitation using this type of whole-genome assay.
Related Questions
- When I get results back, in the transcription factor binding sites, sometimes sequences were colored with blue and red. I wonder what this color means?
- Are transcription factor binding results from the Illumina ChIP Sequencing output quantitative or qualitative?
- How can I identify the binding sites of my transcription factor using ChIP?
