You list sequence comparison and restriction mapping as two ways of identifying introns. What are the differences between the two?
sequence comparison means you go ahead and sequence both the cDNA and the genomic clone of a gene and directly read out the nucleotide sequences of the introns (missing from the cDNA) and the exons (not missing). Restriction mapping basically means that you figure where the restriction sites are in a cDNA and a genomic clone and you look for conserved patterns of restriction sites in both sequences. This can tell you at low resolution where there are big gaps in the genomic clone (introns) but doesn’t tell you what the sequences for each are or exactly where the boundaries between introns and exons lie.
