With the P450-Glo assay kit, what plate format should be used to plate the cryo-plateable hepatocytes?
The cryo-plateable hepatocytes may be plated in any format with a collagen I coating. However, when measuring the luminescence a white opaque polystyrene non-treated flat-bottom 96- or 384-well plates is preferred. (Do not use treated plates, black plates or clear plates.) Sample may need to be transferred from cell culture plate to an opaque, untreated plate depending on your assay design. Make sure that the plate used is compatible with your luminometer.
