With an objective of a given magnification, why can I use eyepieces of increasingly higher magnification to achieve higher total magnification?
To maintain useful magnification yielding satisfactory clarity and resolution, you must avoid empty magnification or making the specimen appear bigger but not clearer. As a general rule, total magnification should not exceed 750X-1000X the NA of the objective. For example, with a 40X, NA 0.65 objective, the total magnification (the multiple of eyepiece and objective magnifications) should lie between 480X and 650X.
Related Questions
- With an objective of a given magnification, why shouldn one use increasingly higher magnification eyepieces to achieve higher total magnification?
- With an objective of a given magnification, why can I use eyepieces of increasingly higher magnification to achieve higher total magnification?
- Why can I use eyepieces of increasingly higher magnification to achieve higher total magnification?