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Will vortexing the cell lysate and cell debris shear genomic DNA and cause genomic DNA contamination?

April 26, 2017cause CELL contamination debris dna genomic lysate shear vortexing

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Will vortexing the cell lysate and cell debris shear genomic DNA and cause genomic DNA contamination?

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AquaPlasmid solution is highly effective in removing genomic DNA contamination. You can easily verify it by using AquaPlasmid kit on E. coli cells that do not harbor plasmids. Vortexing or shaking the lysate and debris ensures cell lysis and plasmid DNA recovery.