Will the software associated with Agilent scanners give data files that can be used with NimbleScan?
It will probably work if the following conditions are met: 1. All images in the TIF must be exactly the same width and height, implying they come from multiple channels of the same scan. There can’t be low res versions or thumbnails in the file. Just quantifiable scans. 2. All “channels” must be digitized as 16 bit grayscale where zero implies no signal and 65535 max signal. 3. To get auto-alignment to work the images must be spatially calibrated. IOW, the size of a pixel is specified in the TIF file. 4. We assume the signal response is linear. IOW we assume twice as bright means twice as much stuff. 5. The images must be spatially linear to within a couple microns or so. We have to be able to lay down a four point alignment and get accurate registration across the field of view.
