Will serum free medium work in the CELLine?
Yes. Many customers report excellent results using serum free medium. The serum free medium is placed on both sides of the semi-permeable membrane in most cases. Importantly, the use of serum free medium may no longer be necessary when the CELLine is used. As the secreted protein is recovered at high concentration from the CELLine, it is no longer necessary to concentrate culture supernatant to recover antibody. This eliminates much of the interference associated with serum protein during purification. At antibody concentrations in excess of 1 mg/ml, some customers apply the cell compartment supernatant directly to an affinity column.
