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Will pipetting error affect the EpiTect Methyl qPCR Array results?

April 26, 2017affect array epitect Error Methyl pipetting qPCR results

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Will pipetting error affect the EpiTect Methyl qPCR Array results?

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The passive reference dyes in the PCR master mixes, such as ROX and Fluorescein, are used by the real-time PCR instruments to normalize variation from well to well. Therefore, these systems tolerate volume variations caused by pipetting error or evaporation. Even a 20% pipetting error causes only 0.05-cycle differences in Ct values.