Will phenol red affect the measurements for alamarBlue®?
There is no interference from the presence of phenol red in the growth medium. The presence of phenol red merely shifts the values approximately 0.03 units higher (see Table 1). Table 1: Effect of phenol red on absorbance values at 570 nm. Absorbance value for various levels of reduction of alamarBlue® in RPMI 1640 pH 7.0, with MOPS (both with and without phenol red) when using Dynatech flat bottom plates and 100μl per well.
