Will Immuno Concepts slides work with other manufacturer s PBS?
Occasionally problems will arise from interactions between another manufacturers reagents and our test systems. Although many vendors sell reagents that appear compatible with our slides, controls, and conjugates, we can offer no guarantees that these alternative products will perform well. Over the years numerous calls have been logged into Immuno Concepts Technical Support regarding artifactual nucleolar patterns, poor or variable staining intensity, film, non-specific high background staining, and mechanical damage. In our experience, many of these problems can be traced back to the use of other manufacturer s reagents with PBS being particularly problematic. The use of alternate PBS can result in an artifactual nucleolar pattern, a generalized weak staining of the substrate, or high-background staining. Most of these problems can be linked to altered pH and ionic strength in other vendors PBS. Dramatic shifts in pH and ionic strength can alter protein conformation and binding chara
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- Will Immuno Concepts slides work with other manufacturer s PBS?
