Will Immuno Concepts slides work with other manufacturer s mounting medium?
Occasionally problems will arise from interactions between another manufacturers reagents and our test systems. Although many vendors sell reagents that appear compatible with our slides, controls, and conjugates, we can offer no guarantees that these alternative products will perform well. Over the years numerous calls have been logged into Immuno Concepts Technical Support regarding artifactual nucleolar patterns, poor or variable staining intensity, film, non-specific high background staining, and mechanical damage. In our experience, many of these problems can be traced back to the use of other manufacturer s reagents with mounting medium being particularly problematic. We have found three common problems associated with other vendor s mounting media: autofluorescence, poor or variable staining intensity, and mechanical damage. Autofluorescence is nonspecific fluorescence given off by the mounting medium itself and generally appears as a film over the entire slide. Poor or variable
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