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Will extra bases in the 5 and 3 UTRs (Untranslated regions) of my gene cloned into a transfer vector affect protein expression?

April 26, 2017bases cloned extra gene regions transfer untranslated UTRs

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Will extra bases in the 5 and 3 UTRs (Untranslated regions) of my gene cloned into a transfer vector affect protein expression?

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A. For optimal protein expression, UTRs have to be as small as possible, especially in 5′ to avoid formation of secondary structures in the mRNA. A minimal length (7-8 bases) in front of the ATG is acceptable, especially if the Kozak consensus sequence (GCCGCCACCATGG) is included. There are no special requirements for the 3’UTR.