Will ammonium ions will inhibit the activity of XbaI and BamHI?
No. According to the book “Experimental Techniques in Bacterial Genetics”, ammonium acetate is actually the best choice for ethanol precipitation of DNA because ammonium is very soluble in ethanol and the salts are efficiently removed with a 70% ethanol wash. Ammonium acetate precipitated samples tend to work best in enzymatic reactions, the exception being for polynucleotide kinase reactions. NaCl and NaAcetate are less soluble in ethanol so will tend to carry over more salt in the sample. However, if the sample contains SDS, NaCl is the precipitant of choice because it helps keep the SDS from co-precipitating with the sample. In other words, if your restriction digest isn’t working, it is not because of the ammonium acetate. Q: One more question is regarding the two enzymes BamH1 and XbaI . How many bases does it occupy in the DNA in search for the recognition sequence? I am trying to digest with BamHi and XbaI using a 73 bp fragment and am not getting the clones I need. A: 1 base fo
