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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?

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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?

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Clumps that occur after addition of Buffer P2 in a bacterial lysate containing LyseBlue reagent indicate poor resuspension of the bacterial cell pellet in Buffer P1. This handling error…

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