Why Utilize Standardized Mixtures of Internal Standards in Quantitative RT-PCR?
There is an urgent need to eliminate the variability of quantitative RT-PCR results obtained by the same samples assayed in different laboratories (7). The cause of the discrepancy is the worker and the reagents. The solution is to utilize standardized mixtures of internal standards to control for the sources of variation. Table 1 outlines several sources of variation in quantitative RT-PCR and the method to control for them.
