Why use gene synthesis instead of PCR cloning?
PCR cloning, the most common method of obtaining genes, may not be able to produce a gene of sufficient quality or even at all. First, a cDNA library for a specific type of tissue has to be prepared or purchased, which requires either time or expense. Second, the gene must be abundant in that cDNA library or it will be very difficult to clone any useful way. Third, when PCR is successful, its products may have mutations or single nucleotide polymorphisms (SNP), which can cause problems during experimentation or other applications. GenScript gene synthesis guarantees delivery of workable quantities of precise, unmutated genes at a far more economical price than PCR cloning. • Is there a length limit for GenScript gene synthesis? A: There is no length limit. GenScript routinely synthesizes genes of 10 kb or longer. • Does codon optimization really matter? A: It matters as much as protein expression matters. Different organisms have different codon usage preferences. For example, the pref
