Why use ChromaLink Biotin or ChromaLink Digoxigenin labeling kits?
For maximum sensitivity and reproducibility it is important to avoid over labeling, which leads to reduced immuno reactivity, or underlabeling, which leads to weak signal. ChromaLink Biotin and Digoxigenin Labeling Kits are the only kits that allow for tracing and quantification of the incorporated label with a simple UV scan of the labeled protein. By simply measuring the Biotin or DIG labeled protein at 280 and 354nm and then inserting those values into an easy to use calculator, you can determine the exact number of biotins or DIG on your antibody.