Why, then, is the use of a large excess of methanol important in the overall reaction?
Since the reaction is equilibrium dependent, the large excess of methanol will drive the reaction toward the synthesis of methyl salicylate and methyl acetate. Methanol is both the solvent and a reactant in the reaction. By the law of mass action, the large amount of methanol forces the reaction toward the right in the reaction as shown in the answer to Question 1. 3. It is reasonable to hypothesize that fungistasis is a proportional phenomenon; that is, exposure to a larger amount of a fungistatic agent will induce greater inhibition than exposure to a lesser amount. How would you test this hypothesis using the bioassay procedure described here? To test if fungistasis is a proportional phenomenon, the experimenter could vary the exposure of a fungus to the fungistatic agent in a systematically increasing manner. For example, using the procedure described in this exercise, a control with no fungistatic agent would be set up, then treatment exposures to 1, 5, 10, 15, etc. microliter amo
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