Why should I incorporate an haemaglutinin (HA) tag sequence?
A frequent problem researchers have is, how am I going to be able to distinguish gene expression produced by my vector from endogenous gene expression especially if endogenous gene expression is high or worse there are no commercial antibodies available to my protein. How do I know the transgene is being expressed? Weve solved this problem for you by including options to incorporate a haemaglutinin (HA) tag sequence either to the N-terminal (i.e. 5) or the C-terminal (i.e. 3) end of your gene of interest. The HA tag enables easy detection and the ability to distinguish endogenous from transgene expression. HA is a commonly used protein surveillance tag. At 10 amino acids in length it has little or no effect on the normal physiological function of the tagged protein, and the presence of this tag on the transgene product of your rAVE vector means through the use of an HA-specific antibody tracking of the protein is simple and efficient. This is extremely useful when using rAVE vectors on
