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Why should DNA or cDNA targets be 100-250 bp long for real-time PCR?

BP cDNA dna PCR real-time targets
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Why should DNA or cDNA targets be 100-250 bp long for real-time PCR?

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Shorter amplification products facilitate high PCR efficiencies. Ideally, amplicon length should be less than 150 bp for optimal amplification efficiency. PCR efficiencies close to 100% are a crucial …

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