Why Sequence Rhizobium leguminosarum?
The productivity of agricultural systems is usually nitrogen dependent. As oil prices rise, so does the price of nitrogen fertilizer, which impacts strongly on global farming economics. The only substitute for fertilizer nitrogen is the use of symbiotic legumes and their nitrogen-fixing rhizobia. This market is worth in excess of US$13 billion globally. The clover rhizobia are amongst the most utilized species of root-nodule bacteria in world agriculture, yet no clover microsymbiont genome has been sequenced. It is thus the goal of this project to completely sequence Rhizobium leguminosarum bv. trifolii (hereafter R. trifolii) using the well characterized, yet dissimilar strains, WSM1325 and WSM2304. Strain WSM1325 is compatible with many perennial clovers of Mediterranean origin used in farming, such as T. pratense, and is therefore one of the most important clover inoculants. However, WSM1325 is incompatible with American and African perennial clovers, such as those nodulated by WSM2