WHY SALIVA vs BLOOD?
HOW HORMONE TESTING WORKS In the past 20-30 years a number of research studies have validated saliva as a diagnostic medium to measure the unbound, biologically active fraction of steroid hormones in the bloodstream. Saliva is a natural ultra-filtrate of blood and steroids not bound by carrier proteins in the blood freely diffuse into saliva. The majority (90-99%) of steroid hormones in the blood are bound tightly to carrier proteins (cortisol-binding globulin, sex-hormone binding globulin, albumin) rendering them unavailable to target tissues. Steroids are very small lipophillic (fat-loving) molecules that, when released from the binding proteins and red blood cells in the blood, freely diffuse into tissues, which include the salivary gland and saliva. The steroid hormones most extensively studied in saliva are Estrogens (Estradiol, Estrone & Estriol), Progesterone, Androgens (DHEAs, Testosterone) & Cortisol for assessment of adrenal function. Saliva measures the free, “bio-available”