Why quantify and characterize exosomes in human body fluids?
Exosomes are small vesicles of endosomal origin with a size of 30-120nm, externally released following the fusion of multivesicular body with the plasma membrane. These structures are secreted from both normal cells and cells from patients with various diseases, including tumors. The importance of exosomes in human pathology is increasing day-by-day not only for their relevance as etiopathological agents in various disease, but also as new possible disease markers needing new approaches for a better quantification and characterization. However, a central problem in obtaining useful in vivo data on these microvesicles is the low level of efficiency of the available methods to obtain exosomes in order to quantify and characterize them from human body fluids, particularly from plasma. Thus, the first goal of this assay is to provide a simple and reliable method to detect and quantify exosomes from human plasma and other body fluids (e.g. ascites, BAL). To view the compete PDF, please clic
