Why purchase my fresh hepatocytes with overlay?
A more three-dimensional, liver-like framework can be modeled in vitro using a Geltrex overlay, which can extend the life of a healthy hepatocyte culture for more chronic applications such as induction or hepatobiliary transport. A Geltrex overlay is typically recommended for time-points more than 48 hours after isolation. A Geltrex overlay would not be recommended for use before transfecting hepatocytes, or for use in protein secretion studies.
