Why not use Plexiglas or Perspex chambers for low-volume?
Some materials, like Plexiglass/Perspex can act as oxygen stores and sinks, forming pools of oxygen which can be released or stored in a reversible way – see Stevens, J. (1992) J. Appl. Physiol. 72, 801-804. This can have an important effect when chambers are down-scaled for micro respirometric measurements of very low oxygen fluxes. Thus, for oxygen measurements in small volumes less than a few milliLitres (ml), we recommend chambers of glas and inert components with non or low oxygen storage capacity! For respirometers with larger volumes (> litre), the use of acrylic materials have no significant effects on measurements.
