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Why must bulls have no contact with sexually mature female cattle for the 10 days just prior to testing?

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Why must bulls have no contact with sexually mature female cattle for the 10 days just prior to testing?

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This down time allows protozoal numbers in the epithelial crypts of the prepuce and penis time to multiply to detectable levels. Bulls may test negative, even if they are actually infected with Tritrichomonas foetus (false negative) without this period of sexual rest.

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