Why iTAg MHC Tetramers?
Modification of the CD8 Binding Region iTAg Tetramers have a proprietary mutation in the alpha-3 domain of the MHC molecule that greatly diminishes nonspecific binding but retains specific binding. The iTAg Tetramer alteration significantly improves binding specificity, providing superior tools for staining and sorting CD8+ T cells. Staining with iTAg Tetramers Staining with iTAg Tetramers allows precise quantitation of antigen-specific T cells by reducing nonspecific CD8/MHC interactions (A). Native Tetramers nonspecifically stain CD8+ lymphocytes in addition to CD8+ antigen-specific lymphocytes, resulting in substantial nonspecific staining (B).* Nonspecific CD8/MHC interactions can be blocked by addition of anti-CD8 antibody, confirming the contribution of these interactions to native Tetramer staining (data not shown). Sorting iTAg Tetramers are more efficient than native Tetramers in sorting specific T cells. Bodinier. Nat Med. 2000;6:707-710. • Column A: Unsorted synovial fluid T
