Why isn phenol miscible in tris buffer?
Your initial premise is wrong. Check out these factsabout phenol: solubility is limited in aliphatic solvents. Phenol’s solubility. In water is limited at room temperature, above 68°C it is entirely water soluble. Solubility in water–Phenol is moderately soluble in water – about 8 g of phenol will dissolve in 100 g of water. If you try to dissolve more than this, you get two layers of liquid. The top layer is a solution of phenol in water, and the bottom one a solution of water in phenol. The solubility behaviour of phenol and water is complicated, and beyond UK A level. Phenol is somewhat soluble in water because of its ability to form hydrogen bonds with the water.
