Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Why is whole-cell PCR recommended for identification of integrants?

April 26, 2017Identification integrants PCR recommended

0

Posted

Why is whole-cell PCR recommended for identification of integrants?

1 Answer

0

Posted

Whole-cell PCR protocols are recommended so that numerous transformants can be rapidly screened simultaneously for integrated (or multiply integrated) expression constructs without the need for purification of genomic DNA from each strain. This dramatically reduces the amount of time needed to test numerous strains for the desired integration event.