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Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?

April 26, 2017CONTROL dna kit negative no-template quantitect transcriptome

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Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?

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In no-template control reactions for the QuantiTect Whole Transcriptome Kit, primer–dimers can form. The highly processive Phi29 DNA polymerase used in the amplification step of the QuantiTect Wh…