Why is the recommended seeding density important?
Cell density has a significant influence on the growth of cells in vitro. E.g. cells secrete a variety of beneficial factors into the media, and as such are able to condition the media in which they are growing. When seeding cells, there is a key level at which the cell density is sufficient for this beneficial effect to function. All media and cells have a minimum cell density below which initial attachment and growth begins to decline. Accordingly, all CELLnTEC media have a recommended minimum seeding density at which extensive tests have confirmed that cells will attach and proliferate well. Conversely, too high seeding densities can also be detrimental, due to the need for frequent passaging, and the difficultly for colonies to form. Find the recommended seeding densities in our protocol page of our resources section.
