Why is the purification of cDNA step optional?
cDNA purification is required for microarray applications. cDNA for fragmentation and labeling reactions and array analysis must be purified to allow for accurate quantitation of cDNA as well as to ensure proper performance of the Encore Biotin Module. If the amplified cDNA is to be used for qPCR analysis alone, then purification is optional. Purification, however, will enable mass normalization of cDNA input into the qPCR reaction, potentially reducing variability.
