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Why is the optimal extension temperature for the HotMaster Taq 68C instead of the usual 72C?

April 26, 2017extension hotmaster optimal taq temperature usual

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Why is the optimal extension temperature for the HotMaster Taq 68C instead of the usual 72C?

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Using modern reaction buffer formulations, Taq polymerases exhibit a maximum activity at 68C and therefore yield even more product than at the traditional value of 72C. Nevertheless, RealMasterMix Probe can be used at 72°C as well.