Why is the Immobilizer better than passive binding?
The Immobilizer binding has several advantages over passive binding to solid phases. One of advantages for the Immobilizer plates and strips is the strong covalent binding of the anthraquinone to the microwell plate. This means that there will be no leaching of the bound molecule. The use of stringent washing procedures and TWEEN® 20 will further avoid unspecific binding in the wells. The coefficient of variation (CV) between the individual wells will also be very low, will give uniform and reproducible results.