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Why is T4gp32 used in the reverse transcription reaction?

April 26, 2017REACTION reverse transcription Used

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Why is T4gp32 used in the reverse transcription reaction?

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T4gp32 is a DNA binding protein that is added to the reverse transcription reaction to increase the efficiency of cDNA synthesis of small amounts of RNA. Since this is a key component in the reverse transcription of RNA samples in the 10pg to 10ng range, it is required in the initial reverse transcription step of the RampUP protocol.