Why is Size Exclusion Chromatography not used in large scale processes?
Size Exclusion Chromatography is a non-adsorptive mode. This means that there is no calculated binding capacity and the amount charged to an SEC column is dependent on the column size, the concentration of the injection, the volume of the injection and the operating flow rate. Typically the size of the column used when scaling up a process requires signicant increases in column size and height which are not economically feasible.
