Why is my antibody not working in ChIP?
There are several reasons: • Antibody-antigen recognition can be significantly affected by the cross-linking step resulting in loss of epitope accessibility and/or recognition. • Use only the antibody of high quality, ChIP-grade. • The best ratio: antibody per amount of chromatin has to be used in ChIP assay. • Do not forget to dilute the sheared chromatin (which contains the SDS) before immunoselection incubation step as it is written in the manual.
