Why is LB broth the most used medium in many academic labs?
LB broth is the most commonly used medium in molecular biology labs for E.coli cell culture. Easy to make, readily available and simple compositions contribute the popularity Of LB broth. In addition, LB broth also has rich nutrient contents. Its osmolarity is close to optimal for E.coli cell growth at early log phase. E.coli strains often grow reasonably fast in LB broth at early log phase. Furthermore, LB broth is sufficient for most molecular biology applications at laboratory scale such as E.coli cell growth and propagation. It is also sufficient for most plasmid, phage, and protein productions at laboratory scales.
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