Why is it recommended to wet the membrane before placing cells into the cell compartment?
It is important to wet the membrane prior to inoculation to assure that the membrane is compliant. The wet membrane is compliant and capable of distension. The dry membrane is stressed significantly if volume is added directly into the cell compartment prior to wetting the membrane. The air trapped in the cell compartment cannot be removed until the membrane is wet and liquid is added into the cell compartment.
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