Why is it important to wash the cells after incubation with a primary antibody?
The washing step is necessary to remove free, unbound primary antibody. If this washing step is not performed, free primary antibody, not bound by the target cell, may interfere with the following magnetic labeling with MACS® MicroBeads. This may lead to a decreased magnetic labeling of target cells and can finally result in a low recovery of positively selected cells.
Related Questions
- The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?
- Can a certain concentration of antibody be recommended for labeling of cells with a primary antibody?
- What are the recommended primary antibody incubation times and temperatures?
