Why is it important to hold the pipette vertically when adding the reaction mix or the sealing solution on the AmpliGrid reaction sites?
The ideal pipetting angle is 70-80°. If the pipetting angle is lower than that, droplet deposition gets inaccurate and the risk for touching the slide surface with the pipette tip is increased. Performing a vertical pipetting results in a nice droplet formation at the end of the pipette tip and a smart deposition of the droplet onto the AmpliGrid reaction site.
